How long do BSA standards last?
III. The BSA Standard should be aliquoted after the first thaw and stored at -20°C. All reagents are stable for up to 12 months under proper storage conditions.
How do you make BSA standards?
Mix 50 μl of the BSA standard solution (2 mg/ml) with 950 μl of diluent and mix well to prepare a 0.1 mg/ml BSA standard solution. Prepare 2 sets of the dilutions of BSA standard solution as shown below 1.5 ml microtubes (7 types x 2 sets = 14 microtubes).
Can BSA go bad?
The diagnosis: BSA, especially when dried and used for blocking, will be fine for days without refrigeration. Powders need to be kept dry more than cold. Powder and stock solutions should also be sturdy for a few days if they’re misplaced over the weekend.
How long does BSA in PBS last?
If the PBS-BSA solution is for blocking it is stable (reliable) for approximately one week at 4c. Should you wish to prepare cytokine aliquots using PBS-BSA I would personally use it immediately or within 2-3 days, before long term storage in -80. Good luck! For immunostaining it works for a week if stored at 4.
How do you find BSA?
The results suggest that FL spectroscopy is the optimum method for determination of BSA in very low concentration, but the use of UV–Vis spectroscopy is simpler and faster in Vis-IR region for BSA detection and determination of its concentration in range of 0.1–2.0 mg/mL.
Is BSA the same as FBS?
BSA is also the main constituent of Fetal Bovine Serum (FBS) and commonly used as a protein concentration standard in laboratory experiments.
How long does a BCA assay take?
Choose the right BCA protein assay for your sample
| Rapid Gold BCA Protein Assay | Micro BCA Protein Assay | |
|---|---|---|
| Incubation time and temperature | 5 min at RT | 60 min at 60°C |
| Total assay time | 50 min | 130 min |
| Absorbance | 480 nm | 562 nm |
| Compatible reagents | Detergents | Detergents |
How do you dilute a BCA standard?
Dilute the contents of one Albumin Standard (BSA) ampule into several clean vials, preferably using the same diluent as the sample(s). Each 1 ml ampule of 2.0 mg/ml Albumin Standard is sufficient to prepare a set of diluted standards for either working range suggested in Table 1.
Can you freeze BSA?
The solution stability of BSA is very good (especially if the solutions are stored as frozen aliquots). In fact, albumins are frequently used as stabilisers for other solubilized proteins (e.g., labile enzymes).
How do you get 2% BSA on PBS?
2% dry skimmed milk in PBS For one plate dissolve 0.2 g of powder in 10 mL of 1X diluted PBS. 5. 1% BSA in PBS For one plate dissolve 0.2 g of BSA in 20 mL of 1X diluted PBS. 6.
Does BSA expire?
Stability. Lyophilized BSA although stable at room temperature for 3 weeks, should be stored 2-8°C. Upon reconstitution BSA should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
What is BSA in a BCA assay?
Pierce BCA Protein Assay Protocol.. Materials. This is the protocol to be used in the determination of total protein in brain tissue by the Pierce BCA method. BSA (Bovine Serum Albumin) is used as the standard and samples are prepared by sonification in 1% (w/v) SDS. REAGENT PREPARATION.
How long do BCA standards last?
12 months
The BCA and Copper Reagents are stable at room temperature. The BSA Standard should be aliquoted after the first thaw and stored at -20°C. All reagents are stable for up to 12 months under proper storage conditions.
