What is the purpose of bromothymol blue in this experiment?
The secret to this experiment is a pH indicator called bromothymol blue. Bromothymol blue is used for managing the pH of fish tanks and pools because it can detect weak acids.
What was bromothymol blue BTB used for in the Elodea experiment?
This lab involves the qualitative measurement of the changes in carbon dioxide concentration associated with respiration and photosynthesis in the freshwater plant Elodea. Bromthymol blue is used as an indicator for the presence of CO2 in solution. When CO2 dissolves in water, carbonic acid is formed.
How does bromothymol blue detect carbon dioxide?
Bromothymol blue is a chemical indicator that changes color as the pH of a solution changes. It is yellow in acidic solutions and blue in basic and neutral solutions. When carbon dioxide is dissolved in water, it creates carbonic acid, pH~5.7. This reaction occurs as rainwater absorbs carbon dioxide.
What is the chemical reaction for bromothymol blue?
The BTB changes color because it functions as an acid/base indicator. The chemical reaction involves carbon dioxide from the teacher’s lungs dissolving in the BTB solution and the water in the solution forming carbonic (weak) acid.
What is the energy of photosynthesis?
photosynthesis, the process by which green plants and certain other organisms transform light energy into chemical energy. During photosynthesis in green plants, light energy is captured and used to convert water, carbon dioxide, and minerals into oxygen and energy-rich organic compounds.
What product of respiration is responsible for the change in color of bromothymol blue solution?
What is the mechanism driving the bromothymol blue solution color change? Bromothymol blue solution is an indicator that changes color in the presence of a weak acid or base. So release of carbon dioxide into water which turns into a weak acid. Therefore the cellular respiration is the driving the color change.
Why does bromothymol blue change color when co2 is added?
When blowing carbon dioxide into a BTB solution, carbonic acid is formed, which changes the pH of the solution and makes it more acidic. As a result, the color of the solution changes from blue to yellow shades.
How does bromophenol blue indicator work?
The bromophenol blue is also used as a dye. At neutral pH, the dye absorbs red light most strongly and transmits blue light. Solutions of the dye therefore are blue. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution.
Which chemical reaction occurs during photosynthesis?
6. Which one chemical reaction occurs during photosynthesis? explanation – the chemical reaction that occurs during photosynthesis means Carbon dioxide is reduced and water is oxidised .
Why is pondweed used for photosynthesis experiments?
Bright light provides more energy than dim light. Red light is more easily used by the pond weed than most other colours. This process of making sugar and oxygen is called photosynthesis. Pond weed uses the carbon dioxide which is dissolved in the water it is in.
Why does bromothymol blue change color when CO2 is added?
Why is bromophenol blue used?
Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis Bromophenol Blue can be used as a color marker and as as a biological stain. Bromophenol Blue can be used to stain proteins and nucleic acids.
What is the purpose of using bromophenol blue?
Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis.
How does bromothymol blue act as an indicator?
Uses of Bromothymol Blue A common demonstration of the pH indicator properties of BTB involves exhaling through a tube into the neutral solution of Bromothymol blue. As the carbon dioxide is absorbed from the breath into the solution, the solution changes its color to yellow from green by forming carbonic acid.
