What is a Michaelis-Menten plot?

This is a plot of the Michaelis-Menten equation’s predicted reaction velocity as a function of substrate concentration, with the significance of the kinetic parameters Vmax and KM graphically depicted.

What does Michaelis-Menten constant tell us?

Km is the Michaelis-Menten constant which shows the concentration of the substrate when the reaction velocity is equal to one half of the maximal velocity for the reaction. It can also be thought of as a measure of how well a substrate complexes with a given enzyme, otherwise known as its binding affinity.

Does lower Km mean higher affinity?

Km may be considered an approximate measure of affinity of an enzyme for its substrate: the lower the Km, the higher is the affinity. At times, optimum conditions cannot be used, and compromises in optimum assay conditions must be made.

What do Vmax and Km tell us?

The importance of determining Km and Vmax If two enzymes, in different pathways, compete for the same substrate, then knowing the values of Km and Vmax for both enzymes permits prediction of the metabolic fate of the substrate and the relative amount that will flow through each pathway under various conditions.

What is the Michaelis-Menten kinetic scheme and how does this explain generally the observed kinetics?

2. What is the Michaelis-Menten kinetic scheme and how does this explain generally the observed kinetics? This scheme generally explains the observed kinetics since it shown the rate being proportional to the amount of E. S whose quantities are proportional to the amount of E and S.

How do you plot a Michaelis-Menten graph?

Using graph paper, draw an x- and y-axis. Label the x-axis mM of [S] or concentration of substrate. Label the y ax- sec/micro-mole of V or velocity of reaction. Insert different values of [S] into the Michaelis-Menten equation, along with the values found for Km and Vmax, to solve for V.

How do you plot a graph Michaelis-Menten?

How do you read a Michaelis-Menten graph?

Explanation: In a classic Michaelis-Menten graph, the y-axis represents reaction rate and the x-axis represents substrate concentration. At low substrate concentrations, the reaction rate increases sharply.

What kind of graph is Michaelis-Menten?

According to Michaelis-Menten kinetics, if the velocity of an enzymatic reaction is represented graphically as a function of the substrate concentration (S), the curve obtained in most cases is a hyperbola.

Why are Michaelis-Menten plots called saturation plots?

Called a SATURATION PLOT or MICHAELIS-MENTEN PLOT after the two biochemists that first described and explained the curve shape. D. Velocity won’t change no matter how much substrate is present. At this point, the enzyme is saturated with substrate, S.

What does the lock and key hypothesis attempts to explain?

Lock-and-key model is a model for enzyme-substrate interaction suggesting that the enzyme and the substrate possess specific complementary geometric shapes that fit exactly into one another. Enzymes are highly specific. They must bind to a specific substrate before they can catalyze a chemical reaction.

What are the main assumptions of the Michaelis-Menten approach to describing enzyme kinetics?

The Michaelis-Menten model for enzyme kinetics presumes a simple 2-step reaction: Step 1: Binding – the substrate binds to the enzyme Step 2: Catalysis – the substrate is converted to product and released (Note that enzymes not matching this reaction scheme may still show similar kinetics.)

What is the primary assumption in Michaelis-Menten enzyme kinetics?

Michaelis-Menten kinetics are characterised by the assumption that the enzyme and substrate participate in an equilibrium with the enzyme-substrate complex which is not disturbed by product formation during the period that the initial rate of reaction is measured.

What are 3 things that can affect the way enzymes work explain how each thing would affect an enzyme?

Factors affecting enzyme activity Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration. Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions can cause an enzyme to lose its ability to bind to a substrate.

Which statement best expresses the information represented in the graph shown?

Cards In This Set

What values do we need to calculate first before we can plot the Michaelis-Menten curve?

The Elements of Life They are carbon, hydrogen, nitrogen, oxygen, phosphorus, and sulfur. The first four of these are the most important, as they are used to construct the molecules that are necessary to make up living cells.