What is LC-MS in toxicology?

What is LC-MS in toxicology?

Toxicology: liquid chromatography mass spectrometry LC-MS is a powerful tool for toxicology applications in the clinical laboratory. The primary use of an LC-MS in a toxicology laboratory is for drug confirmation testing following an immunoassay screen and for broad spectrum drug screening.

How does LC-MS MS work?

The mass spectrometer for an LC/MS/MS system has an ionization source where the LC column effluent is nebulized, desolvated and ionized creating charged particles. These charged particles then migrate under high vacuum through a series of mass analyzers (quadrupole) by applying electromagnetic fields.

What is LCMS water?

Liquid chromatography–mass spectrometry (LC–MS) is a very powerful analytical technique that combines two dimensions of separation techniques. It is finding a rapidly growing demand from water analysis applications due to the increasing need to monitor water for new potential contaminants at very low levels.

How much sample do you need for LC-MS?

Many LC/MS/MS methods only require 50-200 μL of sample. GC/MS methods usually require 1-3 mL of sample. Because of the higher sample volume needed for GC/MS analysis, higher mass sorbent beds in the extraction cartridge are usually needed as well.

What is LCMS grade?

Thermo Scientific Pierce Water, LC-MS Grade is an ultrapure, LC-MS grade preparation with low UV-absorptivity that makes it suitable and reliable for use in HPLC and mass spectrometry applications.

What is LC-MS concentration?

between 1 to 10mg/L
1 mg sample is enough for LC-MS analysis… Actually with LCMS you need very few amount of sample. The concentration you will work with is between 1 to 10mg/L and the injected volume is generally 10microL. The real amount needed is ca 1 micro g.

How much does an LC-MS cost?

between $100 and $200 per sample
The cost per sample is also based upon the number of samples to be analyzed. As a rough approximation metals analyses usually run between $25 and $75 per sample, and LC/MS/MS and GC/MS/MS analyses are typically between $100 and $200 per sample.

What is TIC in LCMS?

A TIC (Total Ion Chromatogram) is a chromatogram created by summing up intensities of all mass spectral peaks belonging to the same scan. The TIC is compared to a GC chromatogram. Note that the TIC includes background noise as well as sample components.

What is the LC-MS method for sterols?

We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and …

What is the LC-MS/MS method for quantification of estradiol and estrone?

New LC-MS/MS bioanalytical method for quantification of Estradiol and Estrone. A new method has been successfully developed and validated for determination of Unconjugated Estradiol and Estrone in human plasma by LC-MS/MS with LLOQ of 2 pg/mL and 5 pg/mL respectively.

How to detect sterols using LC?

Detection of sterols using LC can be by MS, UV, or evaporative light scattering detection (ELSD). The choice of detector is influenced by the sensitivity needed in the separation, with LC/MS/MS providing very high sensitivity.

Why is chromatographic resolution important in the separation of sterols?

As many sterols are positional isomers, chromatographic resolution remains crucial for the analysis because the MS cannot differentiate between the isobaric compounds. Lathosterol and cholesterol are the most challenging compounds in this separation for two reasons: they are difficult to resolve chromatographically, and they are isobaric.

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