What is MS2 coat protein?

The coat protein of the RNA bacteriophage MS2 binds a specific stem-loop structure in viral RNA to accomplish encapsidation of the genome and translational repression of replicase synthesis.

What is the MS2 system?

The MS2-MCP system allows imaging multiple steps of the mRNA life cycle with high temporal and spatial resolution. However for short-lived mRNAs, the tight binding of the MS2 coat protein (MCP) to the MS2 binding sites (MBS) protects the RNA from being efficiently degraded, confounding the study of mRNA regulation.

What is MS2 hairpin?

One of the most widely used tags, MS2, is a 19-nucleotide long viral (bacteriophage) RNA sequence present at the ribosomal binding site of the MS2 replicase mRNA, which folds into a hairpin loop structure.

Is MS2 an antibody?

Recognizes bacteriophage MS2 coat protein. Suitable for Western Blot applications….

How does MS2 tagging work?

MS2 tagging and similar aptamer-based methods involve cloning unique sequences (MS2 hairpins, RNA aptamer sequences, etc.) into the 3′UTR of the transcript of interest; this sequence is then recognized and bound by a fluorescently tagged protein or other molecule using live or fixed cells.

What is RNA aptamer?

RNA Aptamers are defined as RNA oligonucleotides that bind to a specific target with high affinity and specificity, similarly to how an antibody binds to an antigen.

What mutations can FISH detect?

From a medical perspective, FISH can be applied to detect genetic abnormalities such as characteristic gene fusions, aneuploidy, loss of a chromosomal region or a whole chromosome or to monitor the progression of an aberration serving as a technique that can help in both the diagnosis of a genetic disease or suggesting …

What is the structure of the MS2 coat protein?

The structure of the coat protein is a five-stranded β-sheet with two α-helices and a hairpin. When the capsid is assembled, the helices and hairpin face the exterior of the particle, while the β-sheet faces the interior. The MS2 genome is one of the smallest known, consisting of 3569 nucleotides of single-stranded RNA.

What is MS2 genome and gene products?

Genome and gene products. The MS2 genome is one of the smallest known, consisting of 3569 nucleotides of single-stranded RNA. It encodes just four proteins: the maturation protein (A-protein), the lysis protein, the coat protein, and the replicase protein.

Why are short-lived mRNAs tightly bound to MS2 coat proteins?

However, for short-lived mRNAs, the tight binding of the MS2 coat protein (MCP) to the MS2 binding sites (MBS) protects the RNA from being efficiently degraded, and this confounds the study of mRNA regulation.

Can the MS2 aptamer and coat protein be used for RNA localization?

Haimovich, G. et al. Use of the MS2 aptamer and coat protein for RNA localization in yeast: a response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”. RNA 22, 660–666 (2016).