What media is used for CHO cells?
CHO cells should be cultured in Ham’s F12K (ATCC suggestion) or DMEM modified with 10% FBS. If cells are not doubling every 14-17 hours, supplement the medium with 1-2% FCS. Subculture Protocol for CHO: CHO cells grow quickly and easily and cell count should have a doubled within 14-17 hours.
Can cells grow in serum free media?
Serum-free media (SFM) allow researchers to grow a specific cell type or perform a specific application in the absence of serum.
Why is serum free media desirable in cell line protein production?
Wellens: It is indeed desirable to produce proteins in a serum-free environment. It will help reduce risks of virus contamination and it will ease the difficulties encountered in the downstream processing step.
How do CHO cells grow?
CHO Subculture Protocol (adherent) CHO cells grow quickly and easily and cell doubling time is 14-17 hours. Add 3 mL of Trypsin-EDTA to flask and watch for cell layer detachment under an inverted microscope. This should occur within 5-15 minutes. Do not agitate cells during this type as agitation encourages clustering.
Why We Use serum-free media?
Advantages of using serum-free media include a more consistent performance, increased growth and/or productivity, better control over physiological responsiveness, and to reduce risk of contamination by serum-borne adventitious agents in cell culture.
Do CHO cells need serum?
The common CHO cell culture usually requires 10% fetal bovine serum, which can increase the risk of contamination and be unfavorable for protein purification during the downstream process (Kim et al., 2020). Hence, serum-free medium (SFM) for CHO culture is necessary for RTP production.
What is the example of serum-free media?
Serum-free media may contain undefined animal-derived products such as serum albumin (purified from blood), hormones, growth factors, various proteins, etc (2,3).
Why do we need serum-free media?
Serum-free media are media designed to grow a specific cell type or perform a specific application in the absence of serum. The use of serum-free media (SFM) represents an important tool, that allows cell culture to be done with a defined set of conditions as free as possible of confounding variables.
What is serum free media made of?
Types of Serum-Free Media The culture media used in the earliest animal-derived cell cultures were composed of serum (human or fetal bovine), plasma, or tissue extracts of nutrient-like components, which were complex, indefinite composition, and prone to increase the risk of contamination (Lalonde and Durocher, 2017).
What is CHO used for?
CHO cells, which are a cell line derived from the ovary of the Chinese hamster, meant for use in biological and medical research and commercially in the production of therapeutic proteins.
What components are in serum-free media?
Which serum-free media for CHO-K1 cell growth?
In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell … Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production Int J Pharm.
What is serum-free cell culture media?
We are the world’s largest manufacturer of serum-free cell culture media and a leader in the development of innovative specialty media, including serum-free media options. Serum-free media (SFM) allow researchers to grow a specific cell type or perform a specific application in the absence of serum.
Are You Ready to switch to serum-free culture?
Serum-free media (SFM) allow researchers to grow a specific cell type or perform a specific application in the absence of serum. When you’re ready to switch to serum-free culture, we’re ready to help. Whichever adaptation method you choose (sequential or conditioned medium), we strongly recommend that you always take these precautions:
What is the growth rate of Cho dukxb11 cells?
The serum-free formulation supports excellent growth of CHO DUKXB11 cells at low (23cells/cm2) and high (2 x 10(4) cells/cm2) seeding densities characterized by a generation time of 10-12h, and, after addition of 0.2% pluronic F-68, the growth of a recombinant suspension cell line derived from DUKXB11.
