Which detector in HPLC is more sensitive?
Fluorescence HPLC Detectors
Fluorescence HPLC Detectors They are most specific, sensitive and selective, and offers greater sensitivity than the UV-VIS detector. Naturally fluorescent compounds are less as compared to the light absorbing compounds, a limitation solved by post-column derivatization.
What are important characteristics of a detector used in HPLC?
Several features of HPLC Detectors: (1) it should response to all compounds in the mixture ( a general detector) or it should response with known sensitivity (a specific detector). (2) It should not response to mobile phase. (3) It should give linear response to solute concentration.
Which of the following HPLC detector is a bulk property detector?
Refractive Index HPLC detectors: They are also one of the bulk property detectors and are based on the change of the refractive index of the eluent from the column with respect to pure mobile phase .
Which detector is more sensitive?
The UV detector is the most popular providing the highest sensitivity, widest linear dynamic range, and the most stable performance.
What should be ideal characteristics of detector?
Desirable features of a detector High sensitivity. Large linearity of response. Least peak broadening and resultant loss of resolution. Preferably non-destructive.
What is RI detector used for?
A differential refractometer (DRI), or refractive index detector (RI or RID) is a detector that measures the refractive index of an analyte relative to the solvent. They are often used as detectors for high-performance liquid chromatography and size exclusion chromatography.
What is universal detector in HPLC?
A universal detector is defined as the one which ‘can respond to every component in the column effluent except the mobile phase’2. In contrast, selective detectors are defined as ‘detectors which respond to a related group of sample components in the column effluent’.
How do I choose a detector?
The following is a simple guide to selecting a detector: 1….Guidelines for detector selection
- Light power level.
- Wavelength range of incident light.
- Electrical bandwidth of the detector amplifier.
What is the function of detector?
A detector, or radiation transducer, is any device that converts an amount of radiation into some other measurable phenomenon. Ultimately, most of these other measurable phenomena will be tied to an electrical signal. There are two main types of detectors: photon detectors and thermal detectors.
When RI detector is used in HPLC?
HPLC Refractive Index Detectors (HPLC RI Detectors) are used with high-pressure liquid chromatographs when detecting substances with limited or no UV absorption. These chemical components included alcohols, sugars, fatty acids, polymers and carbohydrates.
What is use of RI detector?
What is mass spectroscopy HPLC?
Mass Spectroscopic HPLC Detectors Mass spectroscopy detectors offer very high selectivity and sensitivity and their detection is based on molecular fragmentation by electric fields and separation based on the mass to charge ratio of fragmented molecules.
What are the HPLC detectors used for solute analysis?
The HPLC detectors are used to detect the solute present in the eluent comes from the HPLC column. There are various types of detectors available, but Detector selection in HPLC system is most valuable part in the solute detection. After comparison of all detector, find out which is the best detector for our solute analysis?
What is the difference between HPLC and fixed-wavelength detectors?
HPLC detectors which does not allow to change the wavelength of the radiation called fixed-wavelength detectors. They are usually very cheap, in the nowadays you probably wont be able to find that type of the detector on the market. low-pressure mercury vapor lamp emit very intense light at 253.7 nm.
What are the different types of fluorescent detectors used in HPLC?
There are three types of fluorescent detectors, namely the single wavelength fluorescent detector, multi-wavelength fluorescent detector and laser-induced fluorescent detector. 1. Electrical Conductivity HPLC Detectors They provide reproducible, universal and high sensitivity detection of the charged species and surfactants.