Is HIF-1 a gene or a transcription factor?

Is HIF-1 a gene or a transcription factor?

heterodimeric transcription factor
HIF-1 is a heterodimeric transcription factor consisting of a constitutively expressed β-subunit and an oxygen-regulated α-subunit. The HIF-1α and HIF-1β proteins both contain basic helix-loop-helix motifs that bind DNA and cause subunit dimerization [7,8,9].

What does the EGLN1 gene do?

Normal Function. The EGLN1 gene, often known as PHD2, provides instructions for making an enzyme called prolyl hydroxylase domain 2 (PHD2). The PHD2 enzyme interacts with a protein called hypoxia-inducible factor 2-alpha (HIF-2α).

How many HIF target genes are there?

(A) There are over 70 known direct HIF target genes that function in numerous aspects of both normal physiology and disease processes, including angiogenesis and oxygen supply, stemness/self-renewal, proliferation, epithelial to mesenchymal transition (EMT), metastasis and invasion, redox homeostasis and apoptosis.

What does prolyl hydroxylase do collagen?

Prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline in collagens and other proteins with collagen-like amino acid sequences by the hydroxylation of proline residues in -X-Pro-Glysequences.

Where is proline hydroxylase found?

Hyp is also found in proteins with collagen-like domains, as well as elastin, conotoxins, and argonaute 2. A prolyl hydroxylase domain protein acts on the hypoxia inducible factor α, which plays a key role in sensing molecular oxygen, and could act on inhibitory κB kinase and RNA polymerase II.

What is the HIF-1-alpha transcription factor network?

HIF-1-alpha transcription factor network 23 Reactome pathways for HIF1A Gene BMAL1-CLOCK,NPAS2 activates circadian gene expression Cellular response to hypoxia

What is the function of HIF-1α?

HIF-1α regulates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.

What is the best way to quantify HIF-1 alpha?

If you need to quantify protein, we recommend lysing your cells in RIPA buffer containing protease inhibitors. Because HIF-1 alpha is rapidly degraded in normoxia conditions, we recommend using HIF-1 stabilizers to inhibit degradation. The most used are.

What is the additional pathway information for the HIF1A gene pathway Commons?

Hypoxia Pathway Additional Pathway Information for HIF1A Gene Pathway Commons: Q16665 Interacting Proteins for HIF1A Gene STRING Interaction NetworkPreview (showing top 5 STRING interactants – click image to see top 25)

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