What is dodecyl maltoside?
Dodecyl maltoside (DDM) is a non-ionic detergent that consists of a hydrophilic maltose head and a hydrophobic long chain alkyl tail. It has a relatively low critical micelle concentration of 0.17 mM and is considered a gentle but powerful deter…
Table of Contents
Does Tween 20 lyse cells?
TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.05 to 0.5%. For Laboratory Use Only. Not for drug, household or other uses. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.
How do SDS lyse cells?
Strong ionic detergents such as sodium dodecyl sulphate (SDS) are able to provide cell lysis of the order of seconds, tending to denature proteins from the cell.
What is the CMC of DDM?
DDM (dodecylmaltoside) has a CMC of 0.15 mM and a molecular weight of 510.6.
How do you dissolve cholesteryl hemisuccinate?
A stock solution may be made by dissolving the cholesteryl hemisuccinate in the solvent of choice. Cholesteryl hemisuccinate is soluble in the organic solvent chloroform, which should be purged with an inert gas, at a concentration of approximately 10 mg/ml.
Why do we add SDS to lysis buffer?
Using 1% SDS in lysis buffer ensures complete solubilization and denatures phosphatases.
How do you store a DDM solution?
Store at +4°C.
What does NP-40 stand for?
NP-40 is an ethoxylated nonylphenol for non-ionic surfactants and can act as emulsifier and demulsifier agent. NP-40. Names. Other names. Polyethylene glycol nonylphenyl ether; Nonyl phenoxypolyethoxylethanol; Nonoxynol-40.
Is Tween 80 soluble in water?
Tween 80 is also miscible in water (0.1 ml/ml) and yields a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate, and is practically insoluble in liquid paraffin and fixed oils.
What are the detergents in a lysate buffer?
Lysate buffers contain different detergents that help to release soluble proteins (Triton-X, Tween, SDS, CHAPS). Dependent on the location of the protein of interest, a different lysate buffer is needed to obtain a high yield and purity of the protein.
What is lysis buffer used for?
Preparing Protein Lysates Cell lysis is the breaking down of the cell membrane and the separation of proteins from the non-soluble parts of the cell. Lysate buffers contain different detergents that help to release soluble proteins (Triton-X, Tween, SDS, CHAPS).
Why do I need a different lysate buffer for each protein?
Dependent on the location of the protein of interest, a different lysate buffer is needed to obtain a high yield and purity of the protein. However, every protein is different and may react differently with the buffers and detergents.
What is the product name of lysis buffer 660990lb?
1. Identification of the Substance/Mixture and of the Company/Undertaking Product Code:660990LB Product Name:Lysis Buffer Intended Use:For research use only, not for human or veterinary use. Manufacturer Information Company Name:Cayman Chemical Company