What is FACSCanto?
Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high quality results.
How do you use compensation beads?
In a 5 mL flow tube, add the same volume of your antibody as you use for your stain. Add one drop of positive comp beads and one drop of negative comp beads, or one drop of the all-in-one comp beads.
How do you compensate flow cytometry?
How To Compensate A 4-Color Flow Cytometry Experiment Correctly
- 4 Steps To Compensating A 4-Color Experiment.
- Choose the correct carrier for compensation.
- Step 2: Collect the data and make sure there is a sufficient number of events.
- Calculate compensation correctly.
- Apply the compensation values and inspect the results.
How do TruCount beads work?
TruCount absolute-count tubes contain a lyophilized pellet that dissolves during sample preparation, releasing a known number of fluorescent beads. By gating the bead population during analysis, absolute cell counts can be readily determined by a simple calculation.
What are UltraComp beads?
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent.
What is MFI in flow cytometry?
Mean Fluorescent Intensity (MFI) is often used to compare expression of target of interest (TOI) across samples/ cell populations in Flow cytometry. It gives reliable information about expression/ presence of TOI within the experiment.
Why did my doctor order flow cytometry?
This flow cytometry test is used to diagnose leukemia or lymphoma. This test is usually done after atypical results are seen on a complete blood count or white blood cell (WBC) differential. These results will explain if any abnormal cells are present and what types of cells they are, as a part of your diagnosis.
What are the indications for flow cytometry?
Flow cytometry is most likely to be useful in the following situations: classifying acute leukemias, diagnosing and classifying chronic B cell lymphoproliferative disorders, evaluating T cell and natural killer cell lymphoproliferative disorders, and as an ancillary test in the diagnosis of plasma cell dyscrasias.
What are flow cytometry markers?
These markers are usually functional membrane proteins involved in cell communication, adhesion, or metabolism. Immunophenotyping using flow cytometry has become the method of choice in identifying and sorting cells within complex populations, for example the analysis of immune cells in a blood sample.
