What do you mean by Scissile?
capable of being cut smoothly or split easily
Definition of scissile : capable of being cut smoothly or split easily a scissile peptide bond.
What is a Scissile peptide?
In molecular biology, a scissile bond is a covalent chemical bond that can be broken by an enzyme. Examples would be the cleaved bond in the self-cleaving hammerhead ribozyme or the peptide bond of a substrate cleaved by a peptidase.
How does oxyanion hole work?
An oxyanion hole is a pocket in the active site of an enzyme that stabilizes transition state negative charge on a deprotonated oxygen or alkoxide. The pocket typically consists of backbone amides or positively charged residues.
How does chymotrypsin cleave?
Chymotrypsin cleaves the bond in a sequence of two reactions. In the first reaction, the peptide bond in the substrate is cleaved, and one of the fragments binds covalently to the serine side chain to form anacyl-enzyme intermediate. In the second reaction, this intermediate is cleaved hydrolytically.
How do you name Oxyanions?
- If the oxyanion has the same charge and one oxygen more than the -ate ion then it is named by adding a per- prefix to the name of the -ate ion.
- If the oxyanion has the same charge and one oxygen less than the -ate ion then it is given an -ite ending in place of the -ate ending.
What is the s1 pocket?
The S1 pocket is a deep hydrophobic pocket that allows long, uncharged amino acids like phenylalanine and tryptophan to fit in chymotrypsin. Binding in the S1 pocket positions the adjacent peptide bond at the active site for cleavage.
What structural feature within the active site gives specificity to the protease activity?
A specific pocket adjacent to the active site triad determines the specificity of the protease (chymotrypsin cleaves adjacent to large aromatic side chains, trypsin adjacent to lys or arg residues).
What is the name of oxyanion cio2?
Chlorite is a chlorine oxoanion and a monovalent inorganic anion. It is a conjugate base of a chlorous acid.
What is P1 site?
The substrate residue N-‐terminal to the cleavage site (P1) largely determines the specificity of serine proteases. P1 binds S1, which is called the specificity pocket; its interactions were found early on to be a major determinant of the substrate specificity for trypsin, chymotrypsin and elastase.
Where does subtilisin cleave?
The complex structure of Subtilisin E (1SCJ) is in the intermediate state with the cleaved propeptide still occupying the catalytic pocket. The Loop 100 lines a deep pocket (cave) that accommodates the P2 residue of the substrate (Figure 3A).
How are the enzymes trypsin elastase and chymotrypsin similar?
The serine proteases chymotrypsin, trypsin and elastase, which are produced in an inactive form by the mammalian pancreas, form a closely related group of enzymes. Although only about 40% of the primary structure is common to all three enzymes, most of the catalytically important amino acid residues correspond exactly.
Which type of the substrate specificity is exhibited by the enzymes trypsin and chymotrypsin?
Catalytic mechanisms of these two proteases are similar, but their substrate specificities are different. Trypsin favors basic residues like lysine and arginine; chymotrypsin favors aromatic residues like phenylalanine, tyrosine, and tryptophan (14).
How do you know if it ends in ate or ite?
-ate and -ite are commonly used for polyatomic ions of Oxygen. -ate is used for the ion that has the largest number of Oxygen atoms. The -ite would be used for the ion with the smaller. NO2 and NO3 are known as Nitrite and Nitrate respectively.
What is the meaning of scissile?
Definition of scissile. : capable of being cut smoothly or split easily. a scissile peptide bond.
What is a scissile Gill?
The gills are also scissile, that is, they can be split, and are linear and swollen in the middle. What Do “a.m.” And “p.m.” Stand For?
What does scissile peptide bond mean?
Definition of scissile : capable of being cut smoothly or split easily a scissile peptide bond : capable of being cut smoothly or split easily a scissile peptide bond
What is the origin of the word’scissor’?
[French, from Latin scissilis, from scissus, past participle of scindere, to cut; see scission.] American Heritage® Dictionary of the English Language, Fifth Edition.
