What is a bacterial smear definition?

A bacterial smear is simply that—a small amount of culture spread in a very thin film on the surface of the slide. To prevent the bacteria from washing away during the staining steps, the smear may be chemically or physically “fixed” to the surface of the slide.

What is the purpose of bacterial smear?

The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium. Below are some guidelines for preparing a smear for a Gram-stain. of liquid bacterial growth in the center of a clean slide.

What is bacterial smear preparation?

The first step in most bacterial staining procedures is the preparation of a smear. In a smear preparation, cells from a culture are spread in a thin film over a small area of a microscope slide, dried, and then fixed to the slide by heating or other chemical fixatives.

What is a good bacterial smear?

An ideal bacterial smear should be thin, semi-transparent, whitish layer or film, circular with diameter 1 cm. (approximately) free from dirt, dust or any contamination.

What is smear process?

In a smear preparation, cells from a culture are spread in a thin film over a small area of a microscope slide, dried, and then fixed to the slide by heating or other chemical fixatives. A good smear preparation is the key to a good stain.

What is a bacterial smear quizlet?

What is a bacterial smear? A thin layer of bacteria on a slide for staining.

How many types of smears are there?

Four different types of smear preparation methods (conventional method, blood film method, drop and rest method, and water-wash method) were carried out according to the standard reference as described below. A drop of sample was placed onto a clean slide and then spread to make a smear of 1 cm diameter.

How are bacterial smears made?

Culture from solid media: Using a sterile pipette, add one drop of sterile saline or sterile water to the center of the microscope slide. Aseptically pick a small amount of an isolated colony with a loop and gently mix into the drop of sterile saline or water using circular motions. Mix evenly to make a thin smear.

What is a smear technique?

How is a bacterial smear prepared quizlet?

Terms in this set (5)

1. a sample of a bacterial colony must be mixed into water/saline.
2. using a pipette, apply a small drop of the bacterial fluid to a clean glass slide.
3. smear the fluid evenly across the surface of the slide w/ the edge of a coverslip.
4. turn on Bunsen burner.
5. the slide is now ready for a staining process.

What is the purpose of a smear in microbiology quizlet?

What is the purpose of a bacterial smear? Prepare a sample of bacteria that adheres to a slide. The sample can then be stained so as to better view the bacteria.

What are the types of smear?

Four different types of smear preparation methods (conventional method, blood film method, drop and rest method, and water-wash method) were carried out according to the standard reference as described below.

How is smear prepared?

What is the purpose of the smear preparation?

The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.

What are the two purposes of preparing a smear?

What are the characteristics of a good smear?

A well made blood smear. The classic blood smear has a thumb print appearance. A well developed feathered edge is apparent. The dense body of the smear takes up most of the slide and there is a thin counting area which blends with the feathered edge and the dense body.