What is the purpose of the enzyme catalysis lab?

What is the purpose of the enzyme catalysis lab?

Abstract: The purpose of this lab is to investigate the impact of temperature, substrate concentration, enzyme concentration, and the presence of an inhibitor on the effectiveness and rate of an enzyme. If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well.

What is an enzyme-catalyzed reaction AP Bio?

In an enzyme-catalyzed reaction, the substance to be acted upon, the substrate, binds in the active site of the enzyme. The enzyme and substrate are held together in an enzyme-substrate complex by hydrophobic bonds, hydrogen bonds, and ionic bonds.

Why do you think the instructor cuts the potato into small pieces and why is the blender chilled?

Why do you think the instructor cuts the potato into small pieces and why is the bender chilled? The potato is cut to activate the enzyme and chilled to slow its process.

What are the basic steps of an enzyme-catalyzed reaction?

The basic steps of an enzyme-catalyzed reaction are: substrate and enzyme combine, forming an E-S complex. The reaction occurs, products are released, and the unchanged enzyme is released and recycled.

How substrate concentration affects enzyme activity?

As the substrate concentration increases so does the rate of enzyme activity. An optimum rate is reached at the enzyme’s optimum substrate concentration.

How does crushing the potato affect the enzyme’s activity?

With the large pieces of potatoes, the catalase enzyme were only exposed to the surface of the pieces whereas, the mashed potatoes covered a larger surface area for the enzyme to act upon resulting in greater enzymatic activity.

How does concentration affect the reaction rate of enzymes lab?

Enzyme concentration: Increasing enzyme concentration will speed up the reaction, as long as there is substrate available to bind to. Once all of the substrate is bound, the reaction will no longer speed up, since there will be nothing for additional enzymes to bind to.

How does an enzyme work to catalyze a reaction?

Enzymes (and other catalysts) act by reducing the activation energy, thereby increasing the rate of reaction. The increased rate is the same in both the forward and reverse directions, since both must pass through the same transition state.

What happens during an enzyme catalysed reaction?

To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. These molecules are the enzyme’s substrates. In some reactions, one substrate is broken down into multiple products. In others, two substrates come together to create one larger molecule or to swap pieces.

How does pH affect enzyme-catalyzed?

The rate of enzymatic reaction depends on pH of the medium. Each enzyme has an optimum pH, where the rate of enzymatic reaction is maximum. At higher or lower pH, the rate of an enzymatic reaction decreases. For most enzymes, the optimum pH lies in the range from pH 5 to pH 9.

What affects the rate of enzyme-catalyzed reactions?

Several factors affect the rate at which enzymatic reactions proceed – temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators.

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