What is Botanical Microtechnique?
Botanical microtechnique is an aggregate of methods providing micro visualization of gene and gene product in an entire plant. Plant microtechnique is also a study providing valuable experimental information.
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Which technique can be used for the preparation of tissue of animals and plants?
The tissue culture technique plays an important role in biological studies. The commercially important number of hybrids are routinely propagated by tissue culture technique. It is concluded that the tissue culture technique is of great importance to plant sciences, forestry, and horticulture.
Which method is used mostly for histology of animal tissue?
Staining is the most popular method in histology as one can easily determine the location of a diseased cell by the simple and effective method of staining.
What is microtome in botany?
A microtome (from the Greek mikros, meaning “small”, and temnein, meaning “to cut”) is a cutting tool used to produce extremely thin slices of material known as sections.
What is microtomes in biology?
A microtome is a specialized precision cutting instrument, which accurately and repeatedly slices sections from a block of embedded tissue. Different kinds of microtomes are used to section paraffin and plastic embedded tissues (Figs. 4a–4c) as well as the specialized microtomes used to section frozen tissues (Fig.
Who discovered tissue first?
Xavier Bichat introduced word tissue into the study of anatomy by 1801. He was “the first to propose that tissue is a central element in human anatomy, and he considered organs as collections of often disparate tissues, rather than as entities in themselves”.
What are biological fixatives?
In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing biochemical reactions and may also increase the treated tissues’ mechanical strength or stability.
What is Botanical microtechnique?
Botanical microtechnique is an aggregate of methods providing micro visualization of gene and gene product in an entire plant. Plant microtechnique is also a study providing valuable experimental information.
Which microtechnique is used in both plant and animal micro observation?
Some general microtechnique can be used in both plant and animal micro observation. Whole mounts, smears, squashes, and sections are four commonly used methods when preparing plant and animal specimens for specific purposes.
What are the methods of microtechnique?
Plant microtechnique contains direct macroscopic examinations, freehand sections, clearing, maceration, embedding, and staining. Moreover, three preparation ways used in zoological micro observations are paraffin method, celloidin method, and freezing method. The early development of microtechnique in botany is closely related to that in zoology.
What is zoological microtechnique?
The zoological microtechnique is the art of the preparation for microscopic animal observation. Although many microtechniques can be used in both plant and animal micro experiments. Some methods may differ from itself when employed in different field.
