What is the purpose of immunoelectrophoresis?
Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are proteins that function as antibodies, which fight infection. There are many types of immunoglobulins that fight different types of infections.
What is counter immunoelectrophoresis test?
Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to immunodiffusion, but with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel.
Where is antiserum for immunoelectrophoresis?
Antiserum present in the trough moves toward the antigen components resulting in the formation of separate precipitin lines in 18-24 hrs, each indicating reaction between individual proteins with its antibody. Materials required: Agarose gel, slides, well cutters, Immunoelectrophoresis machine, buffer, etc.
What does abnormal immunological findings in serum mean?
Abnormal test results indicate that there is something affecting the immune system and may suggest the need for further testing. Abnormal Igs are not diagnostic but can, in association with an appropriate clinical history and other tests results, be a strong indicator of a disease or condition.
What happens in counter electrophoresis?
Counter Current Immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody move in opposite directions and form precipitates in the area where they meet in concentrations of optimal proportions.
Which buffer is used in immunoelectrophoresis?
Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies.
How is Rocket immunoelectrophoresis measured?
Procedure of Rocket Immunoelectrophoresis
- Prepare 15 ml of 1 % agarose (as given in important instructions).
- Cool the solution to 55-60oC and add 250 l of antiserum to 13 ml of agarose solution.
- Pour agarose solution containing the antiserum onto a grease free glass plate placed on a horizontal surface.
What is serum protein electrophoresis used for?
Serum protein electrophoresis is used to identify patients with multiple myeloma and other serum protein disorders. Electrophoresis separates proteins based on their physical properties, and the subsets of these proteins are used in interpreting the results.
What does it mean to fix complement?
: the process of binding serum complement to the product formed by the union of an antibody and the antigen for which it is specific that occurs when complement is added to a suitable mixture of such an antibody and antigen and that is the basis of some tests to detect the presence of specific antibodies or antigens.
What are the advantages of Rocket immunoelectrophoresis?
Advantages of Rocket Immunoelectrophoresis Simple, quick, and reproducible method. Several unknown samples can be analyzed on a single plate. Concentrations of proteins as little as 1 µg/mL can be measured requiring as little as 20 ng of protein to be loaded in a well.
Are antigens harmful?
If antigens are perceived as dangerous (for example, if they can cause disease), they can stimulate an immune response in the body. Antigens may be contained within or on bacteria, viruses, other microorganisms, parasites, or cancer cells. Antigens may also exist on their own—for example, as food molecules or pollen.